Yinxin, Zheng and Yong, Yu and Bo, Chen and Huirong, Li (2004) Extracellular enzymatic activities of cold-adapted bacteria from polar oceans and effect of temperature and salinity on cell growth. Advances in Polar Science, 15 (2). pp. 118-128.
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Abstract
The potential of 324 bacteria isolated from different habitats in polar oceans to produce a variety of extracellular enzymatic activities at low temperature was investigated. By plate assay, lipase, protease anylase, gelatinase, agarase, chitinase or cellulase were detected. Lipases were generally present by bacteria living in polar oceans. Protease-producing bacteria held the second highest proportion in culturable isolates. Strains producing anylase kept a relative stable proportion of around 30% in different polarmarine habitats. All 50 arctic sea-ice bacteria producing proteases were cold-adapted strains, however, only 20% were psychrophilic 98% of them could grow at 3% NaCl and 56% could grow without NaCl. On the other hand 98% of these sea-ice bacteria produced extracellular proteases with optimum temperature at or higher than 35°C, well above the upper temperature limit of cell growth. Extracellular enzymes including anylase, agarase, cellulase and lipase released by bacteria from seawater or sediment in polar oceans, most expressed maximum activities between 25 and 35°C. Among extracellular enzymes released by bacterial strain BSw20308, protease expressed maximum activity at 40°C, higher than 35°C of polysaccharide hydrolases and 25°C of lipase.
| Item Type: | Article |
|---|---|
| Related URLs: | |
| Uncontrolled Keywords: | Enzyme, cold-adapted, bacteria, polar ocean. |
| Subjects: | Unspecified |
| Organizations: | Unspecified |
| Date Deposited: | 11 May 2023 10:10 |
| URI: | http://library.arcticportal.org/id/eprint/2279 |
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