Spatial heterogeneity of a microbial community in Kongsfjorden, Svalbard during late summer 2006 and its relationship to biotic and abiotic factors

Chaoying, Guo and Jianfeng, He and Fang, Zhang and Minghong, Cai and Guizhong, Wang (2011) Spatial heterogeneity of a microbial community in Kongsfjorden, Svalbard during late summer 2006 and its relationship to biotic and abiotic factors. Advances in Polar Science, 22 (1). pp. 55-66.

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Abstract

The 16S and 18S ribosomal ribonucleic acid genes of microbial organisms collected from the contrasting environments (temperature, salinity, silicate, phosphate and nitrate, p <0.05) of the inner and outer basins of Kongsfjorden (Spitsbergen, Arctic) were studied using polymerase chain reaction-denaturing gradient gel electrophoresis(DGGE) fingerprinting. Comparison of the microbial fingerprints and the physicochemical parameters revealed that molecular methodology exhibited a greater sensitivity. Sequences obtained from bacterial DGGE were affiliated with four main phylogenetic groups of bacteria: Proteobacteria(Alpha, Beta and Gamma), Bacteroidetes, Verrucomicrobia and Cyanobacteria. The relationships between the genotype distribution of these microbes and associated biotic/abiotic factors, revealed by canonical correspondence analysis, showed that Station 1 at 30 m (outer fjord) was grouped separately from the other sites. This difference could be a consequence of the thermocline and base of the euphotic layer at this depth where the Atlantic and Arctic-type waters overlapped.

Item Type:	Article
Related URLs:
Uncontrolled Keywords:	Microbial community, Genetic diversity, Kongsfjorden, Arctic fjord
Subjects:	Natural Environment > Fauna
Organizations:	Unspecified
Date Deposited:	13 Sep 2023 11:04
URI:	http://library.arcticportal.org/id/eprint/2413

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