Isolation and characteristics of one marine psychrotrophic cellulase - generating bacterium Ar/w/b/75°/10/5 from Chukchi Sea, Arctic

Yinxin, Zheng and Bo, Chen (2002) Isolation and characteristics of one marine psychrotrophic cellulase - generating bacterium Ar/w/b/75°/10/5 from Chukchi Sea, Arctic. Advances in Polar Science, 13 (2). pp. 157-168.

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Abstract

Microorganisms living in polar zones play an important part as the potential source of organic activity materials with low temperature characteristics in the bio-technological applications. A phychrotrophic bacterium (strain Ar/w/b/75°/10/5), producing cellulase at low temperatures during late-exponential and early-stationary phases of cell growth, was isolated from sea ice-covered surface water in Chukchi Sea, Arctic. This bacterium, with rod cells, was Gram-negative, slightly halophilic. Colony growing on agar plate was in black. Optimum growth temperature was 15°C. No cell growth was observed at 35°C or above. Optimum salt concentration for cell growth was between 2 and 3 % of sodium chloride in media. Maximal cellulase activity was detected at a temperature of 35°C and pH8. Cellulase was irreversibly inactivated when incubated at 55°C within 30 min. Enzyme can be kept stable at the temperature no higher than 25°C. Of special interest was that this bacterium produced various extracellular enzymes including cellulase, amylase, agar hydrolase and protease, at low or moderate temperature conditions, which is certainly of it potential value for applications.

Item Type:	Article
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Keywords:	psychrotrophic, bacterium, cellulase, Chukchi Sea, Arctic.
Subjects:	Unspecified
Organizations:	Unspecified
Date Deposited:	08 May 2023 10:07
URI:	http://library.arcticportal.org/id/eprint/2247

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