Isolation of novel psychrophilic bacteria from Arctic sea ice

Yong, Yu and Huirong, Li and Yinxin, Zheng and Bo, Chen (2006) Isolation of novel psychrophilic bacteria from Arctic sea ice. Advances in Polar Science, 17 (2). pp. 90-99.

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Abstract

The phylogenetic diversity of culturable psychrophilic bacteria associated with sea ice from the high latitude regions of Canadian Basin and Chukchi Sea, Arctic, was investigated. A total of 34 psychrophilic strains were isolated using three methods of (iv) dilution plating (at 4 °C), bath culturing (at -1 °C) and dilution plating and cold shock (-20 °C for 24h), bath culturing and dilution plating under aerobic conditions. Sea-ice samples were exposed to -20 °C for 24h that might reduce the number of common microorganisms and encourage outgrowth of psychrophilic strains. This process might be able to be introduced to isolation psychrophilic bacteria from other environmental samples in future study. 16S rDNA nearly full-length sequence analysis revealed that psychrophilic strains felled in two phylogenetic divisions, Y-proteobacteria (in the genera Colwellia Marinobacter Shewanella Glaciecola Marinomonas and Pseudoalteromonas) and Cytophaga-Flexibacter-Bacteroides (Flavobacterium and Psychroflexus). Fifteen of bacterial isolates quite likely represented novel species (16S rDNA sequence similarity below 98%). One of strains (BSi20002) from Canadian Basin showed 100% sequence similarity to that of Marinobacter sp ANT8277 isolated from teh Antarctic Weddell sea ice, suggesting bacteria may have a bipolar distribution at the species level.

Item Type:	Article
Related URLs:
Keywords:	Psychrophilic, Novel bacteria, Sea ice, Arctic
Subjects:	Unspecified
Organizations:	Unspecified
Date Deposited:	13 Jun 2023 11:01
URI:	http://library.arcticportal.org/id/eprint/2310

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