Construction and screening of a functional metagenomic library to identify novel enzymes produced by Antarctic bacteria

Ferrés, Ignacio and Amarelle, Vanesa and Noya, Francisco and Fabiano, Elena (2015) Construction and screening of a functional metagenomic library to identify novel enzymes produced by Antarctic bacteria. Advances in Polar Science, 26 (1). pp. 96-101.

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Abstract

A metagenomic fosmid library of approximately 52 000 clones was constructed to identify functional genes encoding cold-adapted enzymes. Metagenomic DNA was extracted from a sample of glacial meltwater, collected on the Antarctic Peninsula during the ANTARKOS XXIX Expedition during the austral summer of 2012–2013. Each clone contained an insert of about 35–40 kb, so the library represented almost 2 Gb of genetic information from metagenomic DNA. Activity-driven screening was used to detect the cold-adapted functions expressed by the library. Fifty lipase/esterase and two cellulase-producing clones were isolated, and two clones able to grow on Avicel® as the sole carbon source. Interestingly, three clones formed a brown precipitate in the presence of manganese (II). Accumulation of manganese oxides was determined with a leucoberbelin blue assay, indicating that these three clones had manganese-oxidizing activity. To the best of our knowledge, this is the first report of a manganese oxidase activity detected with a functional metagenomic strategy.

Item Type:	Article
Related URLs:
Keywords:	metagenomics, manganese oxidase, esterase, functional screening, cellulase, Antarctica
Subjects:	Unspecified
Organizations:	Unspecified
Date Deposited:	30 Oct 2023 11:09
URI:	http://library.arcticportal.org/id/eprint/2563

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