Structure and function of a novel cold regulated cold shock domain containing protein from an obligate psychrophilic yeast, Glaciozyma antarctica

Charles, Jennifer and Masnoddin, Makdi and Nazaie, Farhan and Yusof, Nur Athirah (2020) Structure and function of a novel cold regulated cold shock domain containing protein from an obligate psychrophilic yeast, Glaciozyma antarctica. Advances in Polar Science, 31 (2). pp. 137-145.

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Abstract

Cold shock domain (CSD)-containing proteins are one of the groups of the evolutionarily conserved nucleic acid-binding proteins in all three domains of life consisting of an ancient beta-barrel fold that serves to bind nucleic acids. The cDNA of a novel protein-coding gene containing CSD was cloned from Glaciozyma antarctica designated as Ga16676. The full length of Ga16676 gene with the size of 1335 bp encodes for an N-terminal CSD with conserved nucleic acids binding motif RNP1 and RNP2. The Ga16676 gene was cloned in pET30 Ek/LIC, sequenced, expressed and its resistance towards cold was characterized. Recombinant protein expression of Ga16676 showed overexpressed soluble expression in both supernatant and pellet forms at 20 ℃. The effects of recombinant CSD protein overexpression on colony formation shows that E. coli cells were able to grow at 37 ℃ and 20 ℃ but not at 4 ℃ while E. coli_Ga16676 cells were able to grow at all temperatures tested. In addition, E. coli_Ga16676 cells showed higher growth rate compared to empty E. coli cells at 10 ℃. Structural analysis of Ga16676 reveals some interesting findings such as more aromatic interactions for efficient binding in low energy environment, a longer loop that may contribute to structural flexibility and clustering of charged amino acids on the protein surface that is important for protein stability and flexibility.

Item Type:	Article
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Keywords:	cold shock protein (CSP), cold adaptation, cold stress, stress response, stress tolerance, Glaciozyma antarctica, psychrophilic yeast
Subjects:	Peoples, Cultures and Societies > Health
Organizations:	Unspecified
Date Deposited:	13 Nov 2023 14:55
URI:	http://library.arcticportal.org/id/eprint/2723

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