Discovery of new polyketide–amino acid conjugates in Antarctic-derived Talaromyces sp. HDN1820200 by overexpression of a pathway-specific transcriptional factor TwnD

Zhang, Xiao and Liu, Luyang and Ma, Chuanteng and Che, Qian and Li, Dehai and Zhu, Tianjiao (2025) Discovery of new polyketide–amino acid conjugates in Antarctic-derived Talaromyces sp. HDN1820200 by overexpression of a pathway-specific transcriptional factor TwnD. Advances in Polar Science, 36 (4). pp. 301-319.

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Abstract

A polyketide synthase–nonribosomal peptide synthetase gene cluster twn in Talaromyces sp. HDN1820200 was activated by overexpression of the pathway-specific transcriptional factor TwnD. Large-scale fermentation and chemical investigation of the mutant strain HDN1820200/TwnD led to the discovery of one new polyketide–amino acid conjugate, bipolamide C and one new polyketide compound, variotin A. The structures of the new compounds were determined by nuclear magnetic resonance (NMR) analysis, high-resolution electrospray ionization mass spectrometry, feeding experiments, NMR calculation and DP4+ analysis. This study revealed that the overexpression of the pathway-specific transcriptional factor represents a promising approach for the discovery of new natural products in fungi within specialized habitat.

Item Type:	Article
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Keywords:	transcription factor overexpression, polyketide–amino acid conjugates, Antarctic fungi, natural product discovery
Subjects:	Natural Environment > Terrestrial
Organizations:	Advances in Polar Science (APS)
Date Deposited:	22 Apr 2026 14:51
URI:	https://library.arcticportal.org/id/eprint/2949

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